anti-dimethyl-histone h3 (lys4) mouse mab

Vapor Pressure :
Recombinant Anti-Dimethyl-Histone H3 (Lys4) Mouse mAb Clone Number: 11130 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K4me2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/Kappa Conjugate Unconjugated Synonyms H3K4me2 UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse IHC-P 1:50 – 1:100 Human, Rat, Mouse ICC/IF 1:50 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones undergo various enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. H3K4me2 is a methylation modification on histone proteins that are tightly linked to DNA. The researchers found that H3K4me2 gradually accumulated after DNA damage occurred and peaked after 24 hours, then gradually declined. The transient elevation of H3K4me2 after DNA damage suggests that it may be involved in the process of DNA damage repair. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: Jurkat, 2: NIH/3T3Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 17 kDaObserved band size: 17 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-Dimethyl-Histone H3 (Lys4) Mouse mAb Clone Number: 11130 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K4me2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/Kappa Conjugate Unconjugated Synonyms H3K4me2 UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse IHC-P 1:50 – 1:100 Human, Rat, Mouse ICC/IF 1:50 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones undergo various enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. H3K4me2 is a methylation modification on histone proteins that are tightly linked to DNA. The researchers found that H3K4me2 gradually accumulated after DNA damage occurred and peaked after 24 hours, then gradually declined. The transient elevation of H3K4me2 after DNA damage suggests that it may be involved in the process of DNA damage repair. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: Jurkat, 2: NIH/3T3Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 17 kDaObserved band size: 17 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with