anti-crotonyl-histone h4 (ser1) rabbit pab

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Anti-Crotonyl-Histone H4 (Ser1) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H4S1cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4S1cr UniProt ID P62805 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:50 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H4 at Ser1 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: JK, Mouse spleenProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 11 kDaObserved band size: 11 kDa ICC/IF Cell line: (A) HeLa, (B) HeLa+Crotonic acid (100ng/ml, 18hr)Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with :
Anti-Crotonyl-Histone H4 (Ser1) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H4S1cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4S1cr UniProt ID P62805 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:50 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H4 at Ser1 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: JK, Mouse spleenProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 11 kDaObserved band size: 11 kDa ICC/IF Cell line: (A) HeLa, (B) HeLa+Crotonic acid (100ng/ml, 18hr)Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with