anti-cox iv rabbit mab

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Recombinant Rabbit Monoclonal Anti-COX IV Rabbit mAb Clone Number: JRMR-10858-114 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P FC IP Reactivity: Human, Mouse, Rat Synonyms: COX4 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms COX4 UniProt ID P13073 Immunogen Recombinant human COX IV fragment MW (kDa) 15 Specificity Anti-COX IV Rabbit mAb recognizes endogeneous level of COX IV protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human FC 1:50 – 1:100 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cytochrome c oxidase IV (COX4) is component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. COX4 is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta. Cellular location Mitochondrion Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Jurkat, 2: MEF, 3: C6, 4: Mouse heartProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 20 kDaObserved band size: 15 kDa IHC-P Tissue: Human liverSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-COX IV Rabbit mAb Clone Number: JRMR-10858-114 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P FC IP Reactivity: Human, Mouse, Rat Synonyms: COX4 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms COX4 UniProt ID P13073 Immunogen Recombinant human COX IV fragment MW (kDa) 15 Specificity Anti-COX IV Rabbit mAb recognizes endogeneous level of COX IV protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human FC 1:50 – 1:100 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cytochrome c oxidase IV (COX4) is component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. COX4 is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta. Cellular location Mitochondrion Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Jurkat, 2: MEF, 3: C6, 4: Mouse heartProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 20 kDaObserved band size: 15 kDa IHC-P Tissue: Human liverSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with