anti-cox iv mouse mab

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Recombinant Anti-COX IV Mouse mAb Clone Number: 10664 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB FC Reactivity: Human Synonyms: COX4I1, COX4, COX4-1, COXIV, COX IV-1, COXIV-1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms COX4I1, COX4, COX4-1, COXIV, COX IV-1, COXIV-1 UniProt ID P13073 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human FC 1:50 – 1:200 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background COXIV, also named as COX4, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV is a marker for mitochondria, which has two isoforms (isoform 1 and 2). Isoform 1 is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COXIV is commonly used as a loading control. Cellular location Mitochondrion Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: A549, 3: HepG2, 4: Jurkat Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 20 kDa Observed band size: 15 kDa FC Cell line: Jurkat Fixative: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary Ab dilution: 1:200 Secondary Ab: Goat Anti-Mouse IgGUnlabeled control: Cells without incubationwith primary and secondary antibodies(Black line).Isotype control: Mouse monoclonal IgG1 (Blueline). Description: Line red is the positive signal for :
Recombinant Anti-COX IV Mouse mAb Clone Number: 10664 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB FC Reactivity: Human Synonyms: COX4I1, COX4, COX4-1, COXIV, COX IV-1, COXIV-1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms COX4I1, COX4, COX4-1, COXIV, COX IV-1, COXIV-1 UniProt ID P13073 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human FC 1:50 – 1:200 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background COXIV, also named as COX4, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV is a marker for mitochondria, which has two isoforms (isoform 1 and 2). Isoform 1 is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COXIV is commonly used as a loading control. Cellular location Mitochondrion Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: A549, 3: HepG2, 4: Jurkat Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 20 kDa Observed band size: 15 kDa FC Cell line: Jurkat Fixative: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary Ab dilution: 1:200 Secondary Ab: Goat Anti-Mouse IgGUnlabeled control: Cells without incubationwith primary and secondary antibodies(Black line).Isotype control: Mouse monoclonal IgG1 (Blueline). Description: Line red is the positive signal for