anti-cleaved parp-1 p85 rabbit mab

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Recombinant Rabbit Monoclonal Anti-Cleaved PARP-1 p85 Rabbit mAb $ 20% off for full 3 pieces –> Clone Number: JRMR-10457-54 Host: Rabbit Clonality: Monoclonal Applications： WB、ICC/IF、FC、IP Reactivity: Human, Mouse, Rat Synonyms: ARTD1, ADPRT, PPOL Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms ARTD1, ADPRT, PPOL UniProt ID P09874 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human, Mouse, Rat ICC/IF 1:100-1:200 Human FC 1:50-1:100 Human, Mouse IP 1:50-1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate, serine or tyrosine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2′-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: 1: HeLa, 2: Jurkat, 3: EL4·IL2, 4: PC_x005f_x0002_12Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 85 kDaObserved MW: 85 kDa ICC/IF Cell line: HeLaFixative: 100% MethanolPermeabilization: 0.1% TritonX-100Primary ab dilution: 1:200Primary incubation condition: 4°C overnightSecondary ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Comment: Color green is the positive signal for :
Recombinant Rabbit Monoclonal Anti-Cleaved PARP-1 p85 Rabbit mAb $ 20% off for full 3 pieces –> Clone Number: JRMR-10457-54 Host: Rabbit Clonality: Monoclonal Applications： WB、ICC/IF、FC、IP Reactivity: Human, Mouse, Rat Synonyms: ARTD1, ADPRT, PPOL Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms ARTD1, ADPRT, PPOL UniProt ID P09874 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human, Mouse, Rat ICC/IF 1:100-1:200 Human FC 1:50-1:100 Human, Mouse IP 1:50-1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate, serine or tyrosine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2′-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: 1: HeLa, 2: Jurkat, 3: EL4·IL2, 4: PC_x005f_x0002_12Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 85 kDaObserved MW: 85 kDa ICC/IF Cell line: HeLaFixative: 100% MethanolPermeabilization: 0.1% TritonX-100Primary ab dilution: 1:200Primary incubation condition: 4°C overnightSecondary ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Comment: Color green is the positive signal for