anti-citrulline-npm1 (arg196) rabbit pab

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Anti-Citrulline-NPM1 (Arg196) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: Numatrin, NMP, NMP1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Numatrin, NMP, NMP1 UniProt ID P06748 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species IHC-P 1:1000 – 1:2000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Nucleophosmin (NPM1) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. NPM1 is involved in ribosomal assembly and rRNA transport. It is highly phosphorylated by Cdc2 kinase during mitosis. Cellular location Nucleus, nucleoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HEK-293T transfected with a control vector containing GFP tag, treatedwith10 mM calcium chloride and 10 µM Ionomycin for 2 hours; (+): HEK-293T transfectedwithGFP-tagged PADI2 (WT) expression vector, treated with 10 mM calciumchlorideand10µM Ionomycin for 2 hours Protein loading amount: 20 μgExposure time: 10 secondsPredicted band size: 33 kDa Observed band size: 38 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HL-60 + DMSO(1.25%, 3-6d), (+): HL-60 + DMSO(1.25%, 3-6d) +A23187(4uM, 15min)Protein loading amount: 20 μgExposure time: 30 secondsPredicted band size: 33 kDa Observed band size: 38 kDa :
Anti-Citrulline-NPM1 (Arg196) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: Numatrin, NMP, NMP1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Numatrin, NMP, NMP1 UniProt ID P06748 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species IHC-P 1:1000 – 1:2000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Nucleophosmin (NPM1) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. NPM1 is involved in ribosomal assembly and rRNA transport. It is highly phosphorylated by Cdc2 kinase during mitosis. Cellular location Nucleus, nucleoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HEK-293T transfected with a control vector containing GFP tag, treatedwith10 mM calcium chloride and 10 µM Ionomycin for 2 hours; (+): HEK-293T transfectedwithGFP-tagged PADI2 (WT) expression vector, treated with 10 mM calciumchlorideand10µM Ionomycin for 2 hours Protein loading amount: 20 μgExposure time: 10 secondsPredicted band size: 33 kDa Observed band size: 38 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HL-60 + DMSO(1.25%, 3-6d), (+): HL-60 + DMSO(1.25%, 3-6d) +A23187(4uM, 15min)Protein loading amount: 20 μgExposure time: 30 secondsPredicted band size: 33 kDa Observed band size: 38 kDa