anti-chmp2b rabbit mab

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Recombinant Rabbit Monoclonal Anti-CHMP2B Rabbit mAb Clone Number: 11092 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: CHMP2.5, CHMP2b, Vps2 – 2, hVps2-2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms CHMP2.5, CHMP2b, Vps2 – 2, hVps2-2 UniProt ID Q9UQN3 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CHMP2B(Charged multivesicular body protein 2b) is a protein that is encoded by the CHMP2B gene in human. It forms part of one of the endosomal sorting complexes required for transport (ESCRT) – specifically ESCRT-III – which are a series of complexes involved in cell membrane remodelling. CHMP2B forms long chains that spiral around the neck of a budding vesicle. Along with the other components of ESCRT-III, CHMP2B constricts the neck of the vesicle just before it is cleaved away from the membrane. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: HEK-293 Protein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 24 kDa Observed band size: 28 kDa IHC-P Tissue: Human liver Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-CHMP2B Rabbit mAb Clone Number: 11092 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: CHMP2.5, CHMP2b, Vps2 – 2, hVps2-2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms CHMP2.5, CHMP2b, Vps2 – 2, hVps2-2 UniProt ID Q9UQN3 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CHMP2B(Charged multivesicular body protein 2b) is a protein that is encoded by the CHMP2B gene in human. It forms part of one of the endosomal sorting complexes required for transport (ESCRT) – specifically ESCRT-III – which are a series of complexes involved in cell membrane remodelling. CHMP2B forms long chains that spiral around the neck of a budding vesicle. Along with the other components of ESCRT-III, CHMP2B constricts the neck of the vesicle just before it is cleaved away from the membrane. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: HEK-293 Protein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 24 kDa Observed band size: 28 kDa IHC-P Tissue: Human liver Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with