anti-cenp-a rabbit mab

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Recombinant Rabbit Monoclonal Anti-CENP-A Rabbit mAb Clone Number: JRMR-10527-48 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: CENPA Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CENPA UniProt ID P49450 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore .The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3.May serve as an epigenetic mark that propagates centromere identity through replication and cell division.Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: A375, 3: HCT-116, 4: Mouse colon, 5: Rat colonProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 16 kDaObserved band size: 18 kDa IHC-P Tissue: Human lymphomaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-CENP-A Rabbit mAb Clone Number: JRMR-10527-48 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: CENPA Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CENPA UniProt ID P49450 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore .The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3.May serve as an epigenetic mark that propagates centromere identity through replication and cell division.Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: A375, 3: HCT-116, 4: Mouse colon, 5: Rat colonProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 16 kDaObserved band size: 18 kDa IHC-P Tissue: Human lymphomaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with