anti-cd9 rabbit mab

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Recombinant Rabbit Monoclonal Anti-CD9 Rabbit mAb Clone Number: JRMR-10172-37B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human Synonyms: MIC3, TSPAN29, GIG2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms MIC3, TSPAN29, GIG2 UniProt ID P21926 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:50 – 1:100 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CD9 antigen is an integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. CD9 is present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, CD9 associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, it associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. It also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. Besides, CD9 is involved in cell adhesion, cell motility and tumor metastasis. Cellular location Membrane, extracellular exosome Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7, 2: HCT-116Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 25 kDaObserved band size: 22 kDa IHC-P Tissue: Human spleenSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-CD9 Rabbit mAb Clone Number: JRMR-10172-37B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human Synonyms: MIC3, TSPAN29, GIG2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms MIC3, TSPAN29, GIG2 UniProt ID P21926 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:50 – 1:100 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CD9 antigen is an integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. CD9 is present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, CD9 associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, it associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. It also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. Besides, CD9 is involved in cell adhesion, cell motility and tumor metastasis. Cellular location Membrane, extracellular exosome Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7, 2: HCT-116Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 25 kDaObserved band size: 22 kDa IHC-P Tissue: Human spleenSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with