anti-cd7 rabbit mab

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Recombinant Rabbit Monoclonal Anti-CD7 Rabbit mAb Clone Number: JRMR-10219 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human Synonyms: T-cell antigen CD7, GP40, TP41 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms T-cell antigen CD7, GP40, TP41 UniProt ID P09564 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The CD7 antigen is a membrane-embedded glycoprotein with a molecular weight of 37-40 kDa and a member of the immunoglobulin supergene family. It plays an important role in T-cell and T-cell/B-cell interactions during early lymphoid development. CD7 is the earliest T-cell-associated molecule to appear in stem cells and prethymic stages and extends its expression all the way to the mature T cells. This molecule is also present on NK cells. In addition, the pluripotent bone marrow stem cells may express CD7. A subpopulation of AML, particularly those with monocytic or megakaryocytic differentiation, may express CD7. Lack of CD7 expression can be used for the detection of T-cell lymphoproliferative disorders, such as mycosis fungoides/Sezary syndrome and adult T-cell leukemia/lymphoma. However, normal and reactive T cells often demonstrate variable degree of CD7 loss. Cellular location Cell membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HepG2, 2: MOLT4 Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 25 kDaObserved band size: 37-40 kDa IHC-P Tissue: Human spleenSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-CD7 Rabbit mAb Clone Number: JRMR-10219 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human Synonyms: T-cell antigen CD7, GP40, TP41 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms T-cell antigen CD7, GP40, TP41 UniProt ID P09564 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The CD7 antigen is a membrane-embedded glycoprotein with a molecular weight of 37-40 kDa and a member of the immunoglobulin supergene family. It plays an important role in T-cell and T-cell/B-cell interactions during early lymphoid development. CD7 is the earliest T-cell-associated molecule to appear in stem cells and prethymic stages and extends its expression all the way to the mature T cells. This molecule is also present on NK cells. In addition, the pluripotent bone marrow stem cells may express CD7. A subpopulation of AML, particularly those with monocytic or megakaryocytic differentiation, may express CD7. Lack of CD7 expression can be used for the detection of T-cell lymphoproliferative disorders, such as mycosis fungoides/Sezary syndrome and adult T-cell leukemia/lymphoma. However, normal and reactive T cells often demonstrate variable degree of CD7 loss. Cellular location Cell membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HepG2, 2: MOLT4 Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 25 kDaObserved band size: 37-40 kDa IHC-P Tissue: Human spleenSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with