anti-acetyl-histone h2b (lys12) rabbit pab

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Anti-Acetyl-Histone H2B (Lys12) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H2BK12ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK12ac UniProt ID P62807 Immunogen Acetylated human histone H2B (Lys12) peptide MW (kDa) 14 Specificity Anti-Acetyl-Histone H2B (Lys12) Rabbit pAb detects histone H2B only when it is acetylated at Lys12. This antibody has been shown to selectively recognize acetylated H2B peptide at Lys12, but not the structurally similar crotonylated peptides at Lys11 or Lys12. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 4 μg per 5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 14 kDa Observed band size: 14 kDa ChIP Cell type: HeLa + sodium butyrate (5 mM, 24 hours) Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 4 μg, 12 μg Beads type and amount per IP: 50 μL of Protein A/G MagBeads Description: Chromatin immunoprecipitations were performed with 1 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human RAB20, GAPDH-CDS, RPL30, LDHA, FOXO3a-promoter, and FOXO3a-downstream regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. :
Anti-Acetyl-Histone H2B (Lys12) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H2BK12ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK12ac UniProt ID P62807 Immunogen Acetylated human histone H2B (Lys12) peptide MW (kDa) 14 Specificity Anti-Acetyl-Histone H2B (Lys12) Rabbit pAb detects histone H2B only when it is acetylated at Lys12. This antibody has been shown to selectively recognize acetylated H2B peptide at Lys12, but not the structurally similar crotonylated peptides at Lys11 or Lys12. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 4 μg per 5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 14 kDa Observed band size: 14 kDa ChIP Cell type: HeLa + sodium butyrate (5 mM, 24 hours) Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 4 μg, 12 μg Beads type and amount per IP: 50 μL of Protein A/G MagBeads Description: Chromatin immunoprecipitations were performed with 1 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human RAB20, GAPDH-CDS, RPL30, LDHA, FOXO3a-promoter, and FOXO3a-downstream regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.