anti-cd45 mouse mab

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Recombinant Anti-CD45 Mouse mAb Clone Number: 10191 Host: Mouse Clonality: Recombinant Monoclonal Applications： FC Reactivity: Human Synonyms: L-CA, PTPRC, Receptor-type tyrosine-protein phosphatase C， T200 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms L-CA, PTPRC, Receptor-type tyrosine-protein phosphatase C， T200 UniProt ID P08575 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species FC 1:50 – 1:200 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CD45, as a phosphatase receptor, is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing. The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates. CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling. Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type. Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression. Cellular location Cell membrane Images FC Cell line: Jurkat Fixative: Unfixed Permeabilization: None Primary Ab dilution: 1:200 Secondary Ab: Goat Anti-Mouse IgGUnlabeled control: Cells without incubationwith primary and secondary antibodies(Black line).Isotype control: Mouse monoclonal IgG1 (Blueline). Description: Line red is the positive signal for :
Recombinant Anti-CD45 Mouse mAb Clone Number: 10191 Host: Mouse Clonality: Recombinant Monoclonal Applications： FC Reactivity: Human Synonyms: L-CA, PTPRC, Receptor-type tyrosine-protein phosphatase C， T200 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms L-CA, PTPRC, Receptor-type tyrosine-protein phosphatase C， T200 UniProt ID P08575 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species FC 1:50 – 1:200 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CD45, as a phosphatase receptor, is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing. The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates. CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling. Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type. Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression. Cellular location Cell membrane Images FC Cell line: Jurkat Fixative: Unfixed Permeabilization: None Primary Ab dilution: 1:200 Secondary Ab: Goat Anti-Mouse IgGUnlabeled control: Cells without incubationwith primary and secondary antibodies(Black line).Isotype control: Mouse monoclonal IgG1 (Blueline). Description: Line red is the positive signal for