anti-cd32b rabbit mab

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Recombinant Rabbit Monoclonal Anti-CD32B Rabbit mAb Clone Number: JRMR-10542-15 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: Fc-gamma RII-b, FCGR2B, FcγRIIB, CD32, FCG2, IGFR2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Fc-gamma RII-b, FCGR2B, FcγRIIB, CD32, FCG2, IGFR2 UniProt ID P31994 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CD32b is receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Daudi, 2: Human prostate cancer, 3: THP-1Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 34 kDaObserved band size: 40-44 kDa IHC-P Tissue: Human spleenSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-CD32B Rabbit mAb Clone Number: JRMR-10542-15 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: Fc-gamma RII-b, FCGR2B, FcγRIIB, CD32, FCG2, IGFR2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Fc-gamma RII-b, FCGR2B, FcγRIIB, CD32, FCG2, IGFR2 UniProt ID P31994 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CD32b is receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Daudi, 2: Human prostate cancer, 3: THP-1Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 34 kDaObserved band size: 40-44 kDa IHC-P Tissue: Human spleenSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with