anti-acetyl-histone h2b (lys11) mouse mab

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Anti-Acetyl-Histone H2B (Lys11) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P ICC/IF IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H2BK11ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK11ac UniProt ID P62807 Immunogen Acetylated human histone H2B (Lys11) peptide MW (kDa) 14 Specificity Anti-Acetyl-Histone H2B (Lys11) Mouse mAb detects histone H2B only when it is acetylated at Lys11. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human IP 1:25 – 1:100 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided below.Lane 1: H2BK11 acetyl. Lane 2: H2BK5 acetyl. Lane 3: H2BK5 butyryl.Lane 4: H2BK5 succinyl. Lane 5: H2BK5 crotonyl. Lane 6: H2BK5 2-hydroxyisobutyryl.Lane 7: H2BK5 β-hydroxybutyryl. Lane 8: H2BK11 butyryl. Lane 9: H2BK11 crotonyl.Lane 10: H2BK11 β-hydroxybutyryl. Lane 11: H2BK12 acetyl. Lane 12: H2BK12 butyryl.Lane 13: H2BK12 crotonyl. Lane 14: H2BK12 2-hydroxyisobutyryl. Lane 15: H2BK12 β-hydroxybutyryl.Lane 16: H2BK11 unmodified. WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:500Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: Mouse liver, Mouse spleenProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa IHC-P Tissue: Human testisSection type: Formalin-fixed & Paraffinembedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Comment: Color brown is the positive signal for :
Anti-Acetyl-Histone H2B (Lys11) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P ICC/IF IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H2BK11ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK11ac UniProt ID P62807 Immunogen Acetylated human histone H2B (Lys11) peptide MW (kDa) 14 Specificity Anti-Acetyl-Histone H2B (Lys11) Mouse mAb detects histone H2B only when it is acetylated at Lys11. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human IP 1:25 – 1:100 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided below.Lane 1: H2BK11 acetyl. Lane 2: H2BK5 acetyl. Lane 3: H2BK5 butyryl.Lane 4: H2BK5 succinyl. Lane 5: H2BK5 crotonyl. Lane 6: H2BK5 2-hydroxyisobutyryl.Lane 7: H2BK5 β-hydroxybutyryl. Lane 8: H2BK11 butyryl. Lane 9: H2BK11 crotonyl.Lane 10: H2BK11 β-hydroxybutyryl. Lane 11: H2BK12 acetyl. Lane 12: H2BK12 butyryl.Lane 13: H2BK12 crotonyl. Lane 14: H2BK12 2-hydroxyisobutyryl. Lane 15: H2BK12 β-hydroxybutyryl.Lane 16: H2BK11 unmodified. WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:500Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: Mouse liver, Mouse spleenProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa IHC-P Tissue: Human testisSection type: Formalin-fixed & Paraffinembedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Comment: Color brown is the positive signal for