anti-cd31 rabbit mab

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Recombinant Rabbit Monoclonal Anti-CD31 Rabbit mAb Clone Number: JRMR-10129-3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP Reactivity: Human, Mouse Synonyms: EndoCAM, GPIIA’, PECA1, PECAM1, PECAM-1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms EndoCAM, GPIIA’, PECA1, PECAM1, PECAM-1 UniProt ID P16284 Immunogen Recombinant human CD31 fragment MW (kDa) 125 Specificity Anti-CD31 Rabbit mAb recognizes endogeneous level of CD31 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:200 – 1:1000 Human ICC/IF 1:50 Human FC 1:50 – 1:100 Human, Mouse IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: THP-1Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 83 kDaObserved band size: 125 kDa IHC-P Tissue: Human cerebrumSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-CD31 Rabbit mAb Clone Number: JRMR-10129-3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP Reactivity: Human, Mouse Synonyms: EndoCAM, GPIIA’, PECA1, PECAM1, PECAM-1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms EndoCAM, GPIIA’, PECA1, PECAM1, PECAM-1 UniProt ID P16284 Immunogen Recombinant human CD31 fragment MW (kDa) 125 Specificity Anti-CD31 Rabbit mAb recognizes endogeneous level of CD31 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:200 – 1:1000 Human ICC/IF 1:50 Human FC 1:50 – 1:100 Human, Mouse IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: THP-1Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 83 kDaObserved band size: 125 kDa IHC-P Tissue: Human cerebrumSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with