anti-cd3 epsilon rabbit mab

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Recombinant Rabbit Monoclonal Anti-CD3 epsilon Rabbit mAb Clone Number: JRMR-42 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P FC IP mIHC-P Reactivity: Human, Mouse, Rat Synonyms: CD3e, T3E Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CD3e, T3E UniProt ID P07766 Immunogen Recombinant human CD3 epsilon fragment MW (kDa) 23 Specificity Anti-CD3 epsilon Rabbit mAb recognizes endogenous level of CD3 epsilon protein. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human IHC-P 1:500 – 1:1200 Human, Mouse, Rat mIHC-P 1:200 – 1:1000 Human FC 1:50 – 1:100 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Cellular location Cytoplasm and membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: JurkatProtein loading quantity: 20 μgExposure time: 3 sPredicted band size: 23 kDaObserved band size: 23 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1200Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-CD3 epsilon Rabbit mAb Clone Number: JRMR-42 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P FC IP mIHC-P Reactivity: Human, Mouse, Rat Synonyms: CD3e, T3E Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CD3e, T3E UniProt ID P07766 Immunogen Recombinant human CD3 epsilon fragment MW (kDa) 23 Specificity Anti-CD3 epsilon Rabbit mAb recognizes endogenous level of CD3 epsilon protein. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human IHC-P 1:500 – 1:1200 Human, Mouse, Rat mIHC-P 1:200 – 1:1000 Human FC 1:50 – 1:100 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Cellular location Cytoplasm and membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: JurkatProtein loading quantity: 20 μgExposure time: 3 sPredicted band size: 23 kDaObserved band size: 23 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1200Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with