anti-acetyl-histone h2a (lys9) rabbit pab

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Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 hours)Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 14 kDa Observed band size: 14 kDa ChIP Cell type: HeLa cells + sodium butyrate (5 mM, 24 hours)Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 4 μg, 12 μgBeads type and amount per IP: 50 μL of Protein A/G MagBeads Description: Chromatin immunoprecipitations were performed with 1 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human RAB20, GAPDH CDS, RPL30, LDHA, FOXO3a promoter, and FOXO3a-downstream regions. The data are presented as enrichment of each sample relative to total amount of input at each amplicon.