anti-acetyl-histone h2a (lys9) rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H2A (Lys9) Rabbit mAb Clone Number: 34H4L2 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IP ChIP Reactivity: Human, Mouse Synonyms: H2AK9ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2AK9ac UniProt ID P04908 Immunogen Acetylated human histone H2A (Lys9) peptide MW (kDa) 14 Specificity Anti-Acetyl-Histone H2A (Lys9) Mouse mAb detects histone H2A only when it is Acetylated at Lys9. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ChIP 6 μg/5×106 cells Human IP 1:25 – 1:100 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Immunogen peptide quantity: 1: 1ng, 4 ng, 16 ng, 64 ng, 2-5: 64 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnight Secondary Ab: (-): MCF-7, (+): MCF-7+ sodiumbutyrate (50mM, 24 hours) + trichostatin A (500ng/ml, 4 hours) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 14 KDa Observed band size: 14 KDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnight Secondary Ab: (-): NIH/3T3, (+): NIH/3T3+ trichostatin A (1μM, 18 hr) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 14 KDa Observed band size: 14 KD ChIP Cell type: MCF-7+Sodium butyrate (50 mM, 24 hr)+trichostatin A (500 ng/ml, 4 hr)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 6 ugBeads type and amount per IP: 50 μl of Protein A MagBeadsComment: The ChIP was performed with 6 μg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH CDS region, RPL30, LDHA CDS region, FOXO3a-promoter, FOXO3adownstream, RAB20 and TuBBP10. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. IP IP of MCF-7+ Sodium butyrate (50 mM, 24hr) +Trichostatin A (500 ng/ml, 4 hr) cells extractsIP ab incubation condition: :
Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H2A (Lys9) Rabbit mAb Clone Number: 34H4L2 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IP ChIP Reactivity: Human, Mouse Synonyms: H2AK9ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2AK9ac UniProt ID P04908 Immunogen Acetylated human histone H2A (Lys9) peptide MW (kDa) 14 Specificity Anti-Acetyl-Histone H2A (Lys9) Mouse mAb detects histone H2A only when it is Acetylated at Lys9. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ChIP 6 μg/5×106 cells Human IP 1:25 – 1:100 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Immunogen peptide quantity: 1: 1ng, 4 ng, 16 ng, 64 ng, 2-5: 64 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnight Secondary Ab: (-): MCF-7, (+): MCF-7+ sodiumbutyrate (50mM, 24 hours) + trichostatin A (500ng/ml, 4 hours) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 14 KDa Observed band size: 14 KDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnight Secondary Ab: (-): NIH/3T3, (+): NIH/3T3+ trichostatin A (1μM, 18 hr) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 14 KDa Observed band size: 14 KD ChIP Cell type: MCF-7+Sodium butyrate (50 mM, 24 hr)+trichostatin A (500 ng/ml, 4 hr)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 6 ugBeads type and amount per IP: 50 μl of Protein A MagBeadsComment: The ChIP was performed with 6 μg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH CDS region, RPL30, LDHA CDS region, FOXO3a-promoter, FOXO3adownstream, RAB20 and TuBBP10. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. IP IP of MCF-7+ Sodium butyrate (50 mM, 24hr) +Trichostatin A (500 ng/ml, 4 hr) cells extractsIP ab incubation condition: