anti-caspase-6 rabbit mab

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Recombinant Rabbit Monoclonal Anti-Caspase-6 Rabbit mAb Clone Number: 11283 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: CASP-6, MCH2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms CASP-6, MCH2 UniProt ID P55212 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Caspase-6 is an Cysteine protease that is encoded by the CASP6 gene in human. During apoptosis, it localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA, thereby inducing nuclear shrinkage and fragmentation. Furthermore, it cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP. In addition, Caspase-6 promotes the ZBP1-mediated activation of programmed cell death pathways including pyroptosis, apoptosis, and necroptosis (PANoptosis) and plays an essential role in defense against viruses. Mechanistically, Caspase-6 interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death. Caspase-6 has known functions in apoptosis, early immune response and neurodegeneration in Huntington’s and Alzheimer’s disease. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: MCF7, 2: SH-SY5Y, 3: Jurkat, 4: A549 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 33 kDa Observed band size: 33 kDa IHC-P issue: Human colon Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Caspase-6 Rabbit mAb Clone Number: 11283 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: CASP-6, MCH2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms CASP-6, MCH2 UniProt ID P55212 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Caspase-6 is an Cysteine protease that is encoded by the CASP6 gene in human. During apoptosis, it localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA, thereby inducing nuclear shrinkage and fragmentation. Furthermore, it cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP. In addition, Caspase-6 promotes the ZBP1-mediated activation of programmed cell death pathways including pyroptosis, apoptosis, and necroptosis (PANoptosis) and plays an essential role in defense against viruses. Mechanistically, Caspase-6 interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death. Caspase-6 has known functions in apoptosis, early immune response and neurodegeneration in Huntington’s and Alzheimer’s disease. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: MCF7, 2: SH-SY5Y, 3: Jurkat, 4: A549 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 33 kDa Observed band size: 33 kDa IHC-P issue: Human colon Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with