anti-casein kinase 2 beta rabbit mab

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Recombinant Rabbit Monoclonal Anti-Casein Kinase 2 beta Rabbit mAb Clone Number: JMMR-1845B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: CK II beta, Protein G5a, Phosvitin Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms CK II beta, Protein G5a, Phosvitin UniProt ID P67870 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse ICC/IF 1:50 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Casein Kinase 2 beta is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit. Cellular location Cytoplasm, Nucleus, secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: 1: HeLa, 2: MCF-7, 3: NIH/3T3, 4: C6Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 25 kDaObserved band size: 25 kDa IHC-P Tissue: Human colon carcinomaSection type: Formalin-fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Casein Kinase 2 beta Rabbit mAb Clone Number: JMMR-1845B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: CK II beta, Protein G5a, Phosvitin Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms CK II beta, Protein G5a, Phosvitin UniProt ID P67870 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse ICC/IF 1:50 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Casein Kinase 2 beta is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit. Cellular location Cytoplasm, Nucleus, secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: 1: HeLa, 2: MCF-7, 3: NIH/3T3, 4: C6Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 25 kDaObserved band size: 25 kDa IHC-P Tissue: Human colon carcinomaSection type: Formalin-fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with