anti-acetyl-alpha tubulin (lys40) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Acetyl-alpha Tubulin (Lys40) Rabbit mAb Clone Number: JRMR-10942-32 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: TUBA1B Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms TUBA1B UniProt ID P68363 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:6000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Rat ICC/IF 1:25 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Cellular location Cytoplasm, Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): MCF-7+serum starvation(14hr), (+): MCF-7+ sodium butyrate (50mM, 24hr) + trichostatin A (500ng/ml, 4 hr)Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 50 kDaObserved band size: 55 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:6000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse brain, 2: Rat brain Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 50 kDaObserved band size: 55 kDa IHC-P Tissue: Human cerebrumSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Acetyl-alpha Tubulin (Lys40) Rabbit mAb Clone Number: JRMR-10942-32 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: TUBA1B Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms TUBA1B UniProt ID P68363 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:6000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Rat ICC/IF 1:25 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Cellular location Cytoplasm, Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): MCF-7+serum starvation(14hr), (+): MCF-7+ sodium butyrate (50mM, 24hr) + trichostatin A (500ng/ml, 4 hr)Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 50 kDaObserved band size: 55 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:6000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse brain, 2: Rat brain Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 50 kDaObserved band size: 55 kDa IHC-P Tissue: Human cerebrumSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with