anti-butyryllysine rabbit pab

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Anti-Butyryllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: All Synonyms: Kbu Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kbu UniProt ID / Immunogen Butyrylated lysine peptides MW (kDa) Multiple Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Butyrylation of lysine, structurally similar to lysine acetylation and lysine propionylation, is a newly identified reversible modification controlling protein activity. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation are widely distributed in wide ranges of prokaryotic and eukaryotic histones and non-histone substrates. Lysine butyrylation may play a vital role in the regulation of transcriptional regulation, tumorigenesis, chromatin dynamics, and plasticity. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 4 ng, 20 ng, 100 ng, 500 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: lane A: PTM301+unmodified BSA peptides, lane B: PTM301+ butyrylated BSA peptides, 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLaProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: Multiple kDaObserved band size: Multiple kDa :
Anti-Butyryllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: All Synonyms: Kbu Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kbu UniProt ID / Immunogen Butyrylated lysine peptides MW (kDa) Multiple Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Butyrylation of lysine, structurally similar to lysine acetylation and lysine propionylation, is a newly identified reversible modification controlling protein activity. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation are widely distributed in wide ranges of prokaryotic and eukaryotic histones and non-histone substrates. Lysine butyrylation may play a vital role in the regulation of transcriptional regulation, tumorigenesis, chromatin dynamics, and plasticity. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 4 ng, 20 ng, 100 ng, 500 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: lane A: PTM301+unmodified BSA peptides, lane B: PTM301+ butyrylated BSA peptides, 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLaProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: Multiple kDaObserved band size: Multiple kDa