Product Name: AFP Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: AFP antibody can be used for detection of AFP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: AFP antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human AFP.The immunogen is located within amino acids 340 – 390 of AFP.
Host Species: Rabbit
Purification: AFP Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 272105-42-7
Product: Disitertide
Buffer: AFP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: AFP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: AFP Antibody: FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein
Accession NO.: NP_001125
Protein Ino: 4501989
Official Symbol: AFP
Geneid: 174
Background: AFP Antibody: Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal development. In adults, AFP expression is often associated with hepatoma or teratoma. However, hereditary persistance of AFP may also be found in individuals with no obvious pathology. AFP is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. AFP levels in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
PubMed ID:http://aac.asm.org/content/52/6/2043.abstract
