anti-butyryl-histone h3 (lys27) mouse mab

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Anti-Butyryl-Histone H3 (Lys27) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: H3K27bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K27bu UniProt ID P68431 Immunogen Butyrylated human histone H3 (Lys27) peptide MW (kDa) 15 Specificity Anti-Butyryl-Histone H3 (Lys27) Mouse mAb detects histone H3 only when it is butyrylated at Lys27. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 17 kDaObserved MW: 17 kDa ICC/IF Cell line:(A) HeLa, (B) HeLa+Sodium butyrate (30mM, 4hr)Fixation:100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°CovernightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Comment: Color green is the positive signal for :
Anti-Butyryl-Histone H3 (Lys27) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: H3K27bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K27bu UniProt ID P68431 Immunogen Butyrylated human histone H3 (Lys27) peptide MW (kDa) 15 Specificity Anti-Butyryl-Histone H3 (Lys27) Mouse mAb detects histone H3 only when it is butyrylated at Lys27. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 17 kDaObserved MW: 17 kDa ICC/IF Cell line:(A) HeLa, (B) HeLa+Sodium butyrate (30mM, 4hr)Fixation:100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°CovernightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Comment: Color green is the positive signal for