anti-butyryl-histone h2b (lys23) mouse mab

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Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) Hela + sodium butyrate (30mM, 4 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa ChIP Cell line: HeLaCross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 6 ugBeads type and amount per IP: 50 μl of Protein G MagBeadsDescription: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human RAB20 and TUBBP10. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.