anti-bmi1 mouse mab

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Recombinant Anti-BMI1 Mouse mAb Clone Number: 12233 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: PCGF4, RNF51 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms PCGF4, RNF51 UniProt ID P35226 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background BMI1 is a protein that is encoded by the BMI1 gene (B cell-specific Moloney murine leukemia virus integration site 1) in human. BMI1 has been reported as an oncogene by regulating p16 and p19, which are cell cycle inhibitor genes. BMI1 gene knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Recently it has been reported BMI1 is rapidly recruited to sites of DNA damage and it sustains for over 8h. BMI1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: MCF7, 2: K-562, 3: A20, 4: NIH/3T3 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 37 kDa Observed band size: 40 kDa :
Recombinant Anti-BMI1 Mouse mAb Clone Number: 12233 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: PCGF4, RNF51 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms PCGF4, RNF51 UniProt ID P35226 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background BMI1 is a protein that is encoded by the BMI1 gene (B cell-specific Moloney murine leukemia virus integration site 1) in human. BMI1 has been reported as an oncogene by regulating p16 and p19, which are cell cycle inhibitor genes. BMI1 gene knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Recently it has been reported BMI1 is rapidly recruited to sites of DNA damage and it sustains for over 8h. BMI1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: MCF7, 2: K-562, 3: A20, 4: NIH/3T3 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 37 kDa Observed band size: 40 kDa