anti-beta-arrestin 1 mouse mab

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Recombinant Anti-beta-Arrestin 1 Mouse mAb Clone Number: JMMR-2675 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: ARRB1, ARR1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms ARRB1, ARR1 UniProt ID P49407 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Beta-Arrestin 1 is one of β-Arrestins (ARRBs), the best-known negative regulators of G protein-coupled receptor signaling. β-Arrestins are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta-Arrestin 1 has been found in various cancers, indicating it is a potential therapeutic target for cancer treatment. Recently, expression of beta-Arrestin 1 in saliva has been identified as a candidate circadian biomarker. Erk1/2 constitutively phosphorylates beta-Arrestin 1 at carboxy-terminal Ser412, which promotes cytosolic localization of the scaffold protein. Dephosphorylation is an essential step of beta-Arrestin 1-mediated receptor endocytosis, but it is not required for receptor desensitization. Cellular location Nucleus, Cytoplasm, Plasma membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: K-562, 2: Jurkat, 3: Mouse brain, 4: Rat brain Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 47 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-beta-Arrestin 1 Mouse mAb Clone Number: JMMR-2675 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: ARRB1, ARR1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms ARRB1, ARR1 UniProt ID P49407 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Beta-Arrestin 1 is one of β-Arrestins (ARRBs), the best-known negative regulators of G protein-coupled receptor signaling. β-Arrestins are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta-Arrestin 1 has been found in various cancers, indicating it is a potential therapeutic target for cancer treatment. Recently, expression of beta-Arrestin 1 in saliva has been identified as a candidate circadian biomarker. Erk1/2 constitutively phosphorylates beta-Arrestin 1 at carboxy-terminal Ser412, which promotes cytosolic localization of the scaffold protein. Dephosphorylation is an essential step of beta-Arrestin 1-mediated receptor endocytosis, but it is not required for receptor desensitization. Cellular location Nucleus, Cytoplasm, Plasma membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: K-562, 2: Jurkat, 3: Mouse brain, 4: Rat brain Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 47 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with