anti-beta tubulin rabbit mab (hrp conjugate)

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Recombinant Rabbit Monoclonal Anti-beta Tubulin Rabbit mAb (HRP Conjugate) Clone Number: JRMR-10943-77 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: TUBB, TUBB5 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate HRP Synonyms TUBB, TUBB5 UniProt ID P07437 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Rat, Mouse IHC-P 1:50 – 1:100 Human, Rat, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins. This antibody is conjugated with HRP. Cellular location Cytoplasm, Cytoskeleton, Microtubule Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Lysate: 1: HeLa, 2: Jurkat, 3: NIH/3T3, 4: PC-12, 5: Mouse brain, 6: Rat brain Protein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 50 kDa Observed band size: 50 kDa IHC-P Tissue: Human tonsil Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-beta Tubulin Rabbit mAb (HRP Conjugate) Clone Number: JRMR-10943-77 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: TUBB, TUBB5 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate HRP Synonyms TUBB, TUBB5 UniProt ID P07437 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Rat, Mouse IHC-P 1:50 – 1:100 Human, Rat, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins. This antibody is conjugated with HRP. Cellular location Cytoplasm, Cytoskeleton, Microtubule Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Lysate: 1: HeLa, 2: Jurkat, 3: NIH/3T3, 4: PC-12, 5: Mouse brain, 6: Rat brain Protein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 50 kDa Observed band size: 50 kDa IHC-P Tissue: Human tonsil Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with