anti-beta ii tubulin rabbit mab

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Recombinant Rabbit Monoclonal Anti-beta II Tubulin Rabbit mAb Clone Number: JRMR-10935-46 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human, Mouse, Rat Synonyms: Tubulin beta-2B chain, TUBB2B Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Tubulin beta-2B chain, TUBB2B UniProt ID Q9BVA1 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Beta-tubulin is a protein that forms the backbone of cells. Tubulin can be divided into several kinds of Tubulin, including α, β, γ, δ, and ε, among which α -tubulin and β -tubulin can form heterodimers and are the two most important Tubulin for microtubule formation. The molecular weights of α-tubulin and β-tubulin are 55kDa and 50kDa, respectively, and the actual detected bands are around 55kDa. As an internal reference protein, the protein levels of β tubulin generally do not change. Therefore, it has been widely used as a reference for the consistency of sample loading in Western Bloting and also for immunostaining to observe the microtubule structure of cells. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: 293T; 2: HepG2; 3: Jurkat; 4: HeLa; 5: NIH/3T3; 6: PC-12 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 50 kDa Observed band size: 55 kDa IHC-P Tissue: Human cerebrumSection type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-beta II Tubulin Rabbit mAb Clone Number: JRMR-10935-46 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human, Mouse, Rat Synonyms: Tubulin beta-2B chain, TUBB2B Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Tubulin beta-2B chain, TUBB2B UniProt ID Q9BVA1 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Beta-tubulin is a protein that forms the backbone of cells. Tubulin can be divided into several kinds of Tubulin, including α, β, γ, δ, and ε, among which α -tubulin and β -tubulin can form heterodimers and are the two most important Tubulin for microtubule formation. The molecular weights of α-tubulin and β-tubulin are 55kDa and 50kDa, respectively, and the actual detected bands are around 55kDa. As an internal reference protein, the protein levels of β tubulin generally do not change. Therefore, it has been widely used as a reference for the consistency of sample loading in Western Bloting and also for immunostaining to observe the microtubule structure of cells. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: 293T; 2: HepG2; 3: Jurkat; 4: HeLa; 5: NIH/3T3; 6: PC-12 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 50 kDa Observed band size: 55 kDa IHC-P Tissue: Human cerebrumSection type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with