anti-beta i tubulin mouse mab

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Recombinant Anti-beta I Tubulin Mouse mAb Clone Number: 10209 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: Tubulin beta-1 chain, TUBB1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Tubulin beta-1 chain, TUBB1 UniProt ID Q9H4B7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Beta-tubulin is a protein that forms the backbone of cells. Tubulin can be divided into several kinds of Tubulin, including α, β, γ, δ, and ε, among which α -tubulin and β -tubulin can form heterodimers and are the two most important Tubulin for microtubule formation. The molecular weights of α-tubulin and β-tubulin are 55kDa and 50kDa, respectively, and the actual detected bands are around 55kDa. Cellular location Cytoplasm, Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: NIH/3T3, 3: Mouse liver, 4: Rat brain, 5: COS-7, 6: PC-12, 7: Neuro-2a, 8: HepG2 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 50 kDa Observed band size: 55 kDa IHC-P Tissue: Human tonsil Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-beta I Tubulin Mouse mAb Clone Number: 10209 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: Tubulin beta-1 chain, TUBB1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Tubulin beta-1 chain, TUBB1 UniProt ID Q9H4B7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Beta-tubulin is a protein that forms the backbone of cells. Tubulin can be divided into several kinds of Tubulin, including α, β, γ, δ, and ε, among which α -tubulin and β -tubulin can form heterodimers and are the two most important Tubulin for microtubule formation. The molecular weights of α-tubulin and β-tubulin are 55kDa and 50kDa, respectively, and the actual detected bands are around 55kDa. Cellular location Cytoplasm, Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: NIH/3T3, 3: Mouse liver, 4: Rat brain, 5: COS-7, 6: PC-12, 7: Neuro-2a, 8: HepG2 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 50 kDa Observed band size: 55 kDa IHC-P Tissue: Human tonsil Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with