Product Name: AES Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, ICC, IF, WB
Applications: AES antibody can be used for the detection of AES by Western blot at 1 – 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: AES antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES.The immunogen is located within the last 50 amino acids of AES.
Host Species: Rabbit
Purification: AES Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 307983-31-9
Product: GRA Ex-25
Buffer: AES Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: AES antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: AES Antibody: GRG, ESP1, GRG5, TLE5, AES-1, AES-2, GRG, Amino-terminal enhancer of split, Gp130-associated protein GAM, Amino enhancer of split
Accession NO.: NP_945320
Protein Ino: 39812019
Official Symbol: AES
Geneid: 166
Background: AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
PubMed ID:http://aac.asm.org/content/52/5/1630.abstract
