anti-atg4b rabbit mab

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Recombinant Rabbit Monoclonal Anti-ATG4B Rabbit mAb Clone Number: JRMR-10671-5 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P FC Reactivity: Human, Mouse, Rat Synonyms: APG4B, AUTL1, KIAA0943 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms APG4B, AUTL1, KIAA0943 UniProt ID Q9Y4P1 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background ATG4B is a member of the autophagin protein family. Its cysteine protease activity is required for the cytoplasm to vacuole transport (Cvt) and autophagy. ATG4B cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: 293T, 2: Ramos, 3: PC-12, 4: Mouse brain, 5: Rat brainProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 44 kDaObserved band size: 44 kDa IHC-P Tissue: Human colon carcinoma_x005f_x0002_Section type: Formalin-fixed & Paraffin_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-ATG4B Rabbit mAb Clone Number: JRMR-10671-5 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P FC Reactivity: Human, Mouse, Rat Synonyms: APG4B, AUTL1, KIAA0943 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms APG4B, AUTL1, KIAA0943 UniProt ID Q9Y4P1 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background ATG4B is a member of the autophagin protein family. Its cysteine protease activity is required for the cytoplasm to vacuole transport (Cvt) and autophagy. ATG4B cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: 293T, 2: Ramos, 3: PC-12, 4: Mouse brain, 5: Rat brainProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 44 kDaObserved band size: 44 kDa IHC-P Tissue: Human colon carcinoma_x005f_x0002_Section type: Formalin-fixed & Paraffin_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with