anti-β-hydroxybutyryl-histone h4 (lys12) rabbit pab

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Anti-β-Hydroxybutyryl-Histone H4 (Lys12) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K12bhb Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K12bhb UniProt ID P62805 Immunogen β-Hydroxybutylated human histone H4 (Lys12) peptide MW (kDa) 11 Specificity Anti-β-Hydroxybutyryl-Histone H4 (Lys12) Rabbit pAb detects histone H4 only when it is β-hydroxybutyrylated at Lys12. This antibody has been shown to selectively recognize β-hydroxybutyrylated H4 peptide at Lys12, but not the acetylated, propionylated, butyrylated, succinylated, or 2-hydroxyisobutyrylated H4 peptide at Lys12. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 6 μg per 5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa ChIP Cell type: HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μgBeads type and amount per IP: 50 μL of Protein A/G MagBeads Description: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human FOXO3a downstream and TUBBP10 regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. :
Anti-β-Hydroxybutyryl-Histone H4 (Lys12) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K12bhb Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K12bhb UniProt ID P62805 Immunogen β-Hydroxybutylated human histone H4 (Lys12) peptide MW (kDa) 11 Specificity Anti-β-Hydroxybutyryl-Histone H4 (Lys12) Rabbit pAb detects histone H4 only when it is β-hydroxybutyrylated at Lys12. This antibody has been shown to selectively recognize β-hydroxybutyrylated H4 peptide at Lys12, but not the acetylated, propionylated, butyrylated, succinylated, or 2-hydroxyisobutyrylated H4 peptide at Lys12. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 6 μg per 5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa ChIP Cell type: HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μgBeads type and amount per IP: 50 μL of Protein A/G MagBeads Description: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human FOXO3a downstream and TUBBP10 regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.