Product Name :
anti-β-hydroxybutyryl-histone h2b (lys15) rabbit pab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H2BK15bhb
UniProt ID :
P62807
Immunogen:
β-Hydroxybutylated human histone H2B (Lys15) peptide
MW (kDa) :
14
Specificity:
Anti-β-Hydroxybutyryl-Histone H2B (Lys15) Rabbit pAb detects histone H2B only when it is β-hydroxybutyrylated at Lys15.
Purity :
Protein A and immunogen affinity purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia. Cellular location Nucleus
Images :
Dot Blot Peptide amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 14 kDa Observed band size: 14 kDa
Vapor Pressure :
Anti-β-Hydroxybutyryl-Histone H2B (Lys15) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2BK15bhb Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（2） $ 435 Recombinant Rabbit Monoclonal Anti-β-Hydroxybutyryl-Histone H2B (Lys15) Rabbit mAb Catalog #
Anti-β-Hydroxybutyryl-Histone H2B (Lys15) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2BK15bhb Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（2） $ 435 Recombinant Rabbit Monoclonal Anti-β-Hydroxybutyryl-Histone H2B (Lys15) Rabbit mAb Catalog #
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