anti-xlf mouse mab

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Recombinant Anti-XLF Mouse mAb Clone Number: JMMR-2071 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: NHEJ1, Protein cernunnos, XRCC4-like factor Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms NHEJ1, Protein cernunnos, XRCC4-like factor UniProt ID Q9H9Q4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Non-homologous end-joining factor 1 (NHEJ1), also known as Cernunnos or XRCC4-like factor (XLF), is a protein that is encoded by the NHEJ1 gene in human. XLF was originally discovered as the protein mutated in five patients with growth retardation, microcephaly, and immunodeficiency. The protein is required for the non-homologous end joining (NHEJ) pathway of DNA repair. XLF interacts with DNA ligase IV and XRCC4 and is thought to be involved in the end-bridging or ligation steps of NHEJ. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: HEK-293, 3: Raji Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 33 kDa Observed band size: 37 kDa IHC-P Tissue: Human endometrium carcinoma Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-XLF Mouse mAb Clone Number: JMMR-2071 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: NHEJ1, Protein cernunnos, XRCC4-like factor Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms NHEJ1, Protein cernunnos, XRCC4-like factor UniProt ID Q9H9Q4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Non-homologous end-joining factor 1 (NHEJ1), also known as Cernunnos or XRCC4-like factor (XLF), is a protein that is encoded by the NHEJ1 gene in human. XLF was originally discovered as the protein mutated in five patients with growth retardation, microcephaly, and immunodeficiency. The protein is required for the non-homologous end joining (NHEJ) pathway of DNA repair. XLF interacts with DNA ligase IV and XRCC4 and is thought to be involved in the end-bridging or ligation steps of NHEJ. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: HEK-293, 3: Raji Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 33 kDa Observed band size: 37 kDa IHC-P Tissue: Human endometrium carcinoma Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with