anti-v5 tag mouse mab

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Recombinant Anti-V5 Tag Mouse mAb This item is discontinued. Please see alternative products below. Clone Number: JMMR-2652 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: / Synonyms: / Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms / UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:20000 / Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background V5-tag (amino acid sequence: GKPIPNPLLGLDST) is a polypeptide consisting of amino acid residues 95-108 isolated from the P and V proteins of simian virus 5 (SV5). V5 tags are often fused to the N-terminus or C-terminus of target proteins for analysis and identification of target proteins. Cellular location / Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000/1:2000/1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: 1: Saccharomyces cerevisiae transfected with an empty vector (vectorcontrol), whole cell lysate ; 2: Saccharomyces cerevisiae transfected with human Snx4 (WT)(57 kDa) expression vector containing four V5-tags,whole cell lysateExposure time: 3 sObserved band size: 57 kDa :
Recombinant Anti-V5 Tag Mouse mAb This item is discontinued. Please see alternative products below. Clone Number: JMMR-2652 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: / Synonyms: / Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms / UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:20000 / Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background V5-tag (amino acid sequence: GKPIPNPLLGLDST) is a polypeptide consisting of amino acid residues 95-108 isolated from the P and V proteins of simian virus 5 (SV5). V5 tags are often fused to the N-terminus or C-terminus of target proteins for analysis and identification of target proteins. Cellular location / Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000/1:2000/1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: 1: Saccharomyces cerevisiae transfected with an empty vector (vectorcontrol), whole cell lysate ; 2: Saccharomyces cerevisiae transfected with human Snx4 (WT)(57 kDa) expression vector containing four V5-tags,whole cell lysateExposure time: 3 sObserved band size: 57 kDa