anti-ubiquitin rabbit mab (n-terminal)

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Recombinant Rabbit Monoclonal Anti-Ubiquitin Rabbit mAb (N-terminal) Clone Number: 14H4L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID P62979 Immunogen Synthetic peptide corresponding to the N-terminus of human ubiquitin MW (kDa) Multiple Specificity Anti-Ubiquitin Rabbit mAb (N-terminal) detects endogenous ubiquitin, polyubiquitin, and ubiquitinated proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:50 – 1:200 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating cellular processes. By covalently attaching to target proteins through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitination marks the target proteins for proteasomal degradation, modulates membrane protein trafficking, alters protein-protein interactions, and controls the activity of many signal transduction pathways. Ubiquitination occurs through the formation of an isopeptide bond between its C-terminal Gly76 and a lysine residue in the target protein. This process can occur either as a monomer (monoubiquitin) or as a polymer (polyubiquitin chains), where the C-terminus of a chain extending ubiquitin becomes linked to the N-terminus (M1) or one of seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) within a substrate-bound ubiquitin molecule, resulting in polyubiquitin chains with different functions. Lys6-linked may be involved in DNA repair; Lys11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys29-linked is involved in lysosomal degradation; Lys33-linked is involved in kinase modification; Lys48-linked is involved in protein degradation via the proteasome; Lys63-linked is involved in endocytosis, and DNA-damage responses. Cellular location Nucleus, Cytoplasm Images WB Lysate: HeLa, MCF-7, BRL, NIH/3T3 Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds Predicted band size: Multiple Observed band size: Multiple ICC/IF Cell line: HeLaFixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:200Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Ubiquitin Rabbit mAb (N-terminal) Clone Number: 14H4L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID P62979 Immunogen Synthetic peptide corresponding to the N-terminus of human ubiquitin MW (kDa) Multiple Specificity Anti-Ubiquitin Rabbit mAb (N-terminal) detects endogenous ubiquitin, polyubiquitin, and ubiquitinated proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:50 – 1:200 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating cellular processes. By covalently attaching to target proteins through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitination marks the target proteins for proteasomal degradation, modulates membrane protein trafficking, alters protein-protein interactions, and controls the activity of many signal transduction pathways. Ubiquitination occurs through the formation of an isopeptide bond between its C-terminal Gly76 and a lysine residue in the target protein. This process can occur either as a monomer (monoubiquitin) or as a polymer (polyubiquitin chains), where the C-terminus of a chain extending ubiquitin becomes linked to the N-terminus (M1) or one of seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) within a substrate-bound ubiquitin molecule, resulting in polyubiquitin chains with different functions. Lys6-linked may be involved in DNA repair; Lys11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys29-linked is involved in lysosomal degradation; Lys33-linked is involved in kinase modification; Lys48-linked is involved in protein degradation via the proteasome; Lys63-linked is involved in endocytosis, and DNA-damage responses. Cellular location Nucleus, Cytoplasm Images WB Lysate: HeLa, MCF-7, BRL, NIH/3T3 Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds Predicted band size: Multiple Observed band size: Multiple ICC/IF Cell line: HeLaFixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:200Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with