anti-ttf1 rabbit mab

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Recombinant Rabbit Monoclonal Anti-TTF1 Rabbit mAb Clone Number: JRMR-10821-58B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, NKX2-1, NKX2A, TTF-1, T/EBP, Thyroid Transcription Factor-1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, NKX2-1, NKX2A, TTF-1, T/EBP, Thyroid Transcription Factor-1 UniProt ID P43699 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Transcription termination factor 1(TTF-1) is an essential protein that controls the RNA Polymerase I transcription of the rDNA genes. TTF1 binds to the Sal Box, a repetitive sequence element in the spacer region downstream of the 3’ ends of the genes. Binding of TTF1 to this element can pause the elongation complex through the activity of chromatin remodeling complexes, such as NoRC. TTF1 also has an activating function by binding an upstream terminator element to induce chromatin remodeling. TTF1 localization can be controlled by key tumor suppressor ARF, which binds to the TTF1 nucleolar localization sequence, sending it to the nucleoplasm. This process drastically reduces rRNA synthesis. Furthermore, TTF1 can be ubiquitinylated by MDM2, which targets it for degradation. TTF1 has also been identified to play a role in HIV-1 replication. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: A549 (Negative control), 2: TTProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 42 kDaObserved band size: 42 kDa IHC-P Tissue: Human lungSection type: Formalin-fixed & paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and MousePolymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-TTF1 Rabbit mAb Clone Number: JRMR-10821-58B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, NKX2-1, NKX2A, TTF-1, T/EBP, Thyroid Transcription Factor-1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, NKX2-1, NKX2A, TTF-1, T/EBP, Thyroid Transcription Factor-1 UniProt ID P43699 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Transcription termination factor 1(TTF-1) is an essential protein that controls the RNA Polymerase I transcription of the rDNA genes. TTF1 binds to the Sal Box, a repetitive sequence element in the spacer region downstream of the 3’ ends of the genes. Binding of TTF1 to this element can pause the elongation complex through the activity of chromatin remodeling complexes, such as NoRC. TTF1 also has an activating function by binding an upstream terminator element to induce chromatin remodeling. TTF1 localization can be controlled by key tumor suppressor ARF, which binds to the TTF1 nucleolar localization sequence, sending it to the nucleoplasm. This process drastically reduces rRNA synthesis. Furthermore, TTF1 can be ubiquitinylated by MDM2, which targets it for degradation. TTF1 has also been identified to play a role in HIV-1 replication. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: A549 (Negative control), 2: TTProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 42 kDaObserved band size: 42 kDa IHC-P Tissue: Human lungSection type: Formalin-fixed & paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and MousePolymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with