Product Name :
anti-trimethyl-histone h3 (lys23) rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H3K23me3
UniProt ID :
P68431
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, Trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus
Images :
Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours atroom temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 4 ng,16 ng, 64ngExposure time: 10 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Rat kidney, 5: Mouse kidney, 6: Recombinant histone H3 (20ng)Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 15 kDaObserved band size: 15 kDa FC Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 90% methanolPrimary Ab dilution: 1:100Secondary Ab: Goat anti Rabbit IgGUnlabeled control: Cells without incubation with primary and secondary antibodies (Black line).Isotype control: Rabbit monoclonal IgG (Blue line).Description: Line red is the positive signal for
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Trimethyl-Histone H3 (Lys23) Rabbit mAb This item is discontinued. Please see alternative products below. Clone Number: PT
Recombinant Rabbit Monoclonal Anti-Trimethyl-Histone H3 (Lys23) Rabbit mAb This item is discontinued. Please see alternative products below. Clone Number: PT
Related websites: https://www.medchemexpress.com/antibodies.html
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