anti-tprkb mouse mab

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Recombinant Anti-TPRKB Mouse mAb Clone Number: JMMR-2748 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: PRPK-binding protein, CGI-121, My019 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms PRPK-binding protein, CGI-121, My019 UniProt ID Q9Y3C4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:4000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background TPRKB is a component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TPRKB acts as an allosteric effector that regulates the t6A activity of the complex. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:4000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: Caco-2, 2: Raji, 3: A549, 4: JurkatProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 20 kDaObserved band size: 20 kDa ICC/IF Cell line: Caco-2Fixative: 100% Ice-cold methanolPermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Anti-TPRKB Mouse mAb Clone Number: JMMR-2748 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: PRPK-binding protein, CGI-121, My019 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms PRPK-binding protein, CGI-121, My019 UniProt ID Q9Y3C4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:4000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background TPRKB is a component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TPRKB acts as an allosteric effector that regulates the t6A activity of the complex. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:4000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: Caco-2, 2: Raji, 3: A549, 4: JurkatProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 20 kDaObserved band size: 20 kDa ICC/IF Cell line: Caco-2Fixative: 100% Ice-cold methanolPermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with