anti-ape1 rabbit mab

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Recombinant Rabbit Monoclonal Anti-APE1 Rabbit mAb Clone Number: JRMR-10907-27 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human, Mouse, Rat Synonyms: Ape, Apex, Ref1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Ape, Apex, Ref1 UniProt ID P28352 Immunogen Recombinant human APE1 fragment MW (kDa) 37 Specificity Anti-APE1 Rabbit mAb recognizes endogeneous level of APE1 protein. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse IHC-P 1:200 – 1:1000 Human, Mouse, Rat IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background APEX encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HEK-293, 2: HepG2, 3: NIH/3T3, 4: RAW264.7Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 35 kDaObserved band size: 37 kDa IHC-P Tissue: Human ovarian carcinomaSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-APE1 Rabbit mAb Clone Number: JRMR-10907-27 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human, Mouse, Rat Synonyms: Ape, Apex, Ref1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Ape, Apex, Ref1 UniProt ID P28352 Immunogen Recombinant human APE1 fragment MW (kDa) 37 Specificity Anti-APE1 Rabbit mAb recognizes endogeneous level of APE1 protein. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse IHC-P 1:200 – 1:1000 Human, Mouse, Rat IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background APEX encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HEK-293, 2: HepG2, 3: NIH/3T3, 4: RAW264.7Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 35 kDaObserved band size: 37 kDa IHC-P Tissue: Human ovarian carcinomaSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with