Product Name: Adipolean Antibody
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:To detect hgAcrp30/Adipolean by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 – 0.4 ng/well of recombinant hgAcrp30/Adipolean. Western Blot:To detect hgAcrp30/Adipolean by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hgAcrp30/Adipolean is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL
Predicted Molecular Weight:
Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hgAcrp30/Adipolean (human gAcrp30/Adipolean).
Host Species: Goat
Purification:
Physical State: Lyophilized
CAS NO.: 216450-65-6
Product: Sophoflavescenol
Buffer:
Concentration:
Storage Conditions: Adipolean antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ACDC, ADPN, APM1, APM-1, GBP28, ACRP30, ADIPQTL1, ACDC, Adiponectin, 30 kDa adipocyte complement-related protein
Accession NO.: Q15848
Protein Ino: 2493789
Official Symbol: ADIPOQ
Geneid: 9370
Background: Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.
PubMed ID:http://aac.asm.org/content/52/4/1401.abstract
