anti-syvn1 mouse mab

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Recombinant Anti-SYVN1 Mouse mAb Clone Number: 11373 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: HRD1, KIAA1810 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms HRD1, KIAA1810 UniProt ID Q86TM6 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 Human IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background E3 ubiquitin-protein ligase synoviolin is an enzyme that in humans is encoded by the SYVN1 gene. This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. This gene and the mitochondrial ribosomal protein L49 gene use in their respective 3′ UTRs some of the same genomic sequence. Sequence analysis identified two transcript variants that encode different isoforms. Cellular location Endoplasmic reticulum, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:3000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: A549, 2: HeLa, 3: HEK-293, 4: HepG2Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 68 kDaObserved band size: 70 kDa IHC-P Tissue: Human liver cancerSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-SYVN1 Mouse mAb Clone Number: 11373 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: HRD1, KIAA1810 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms HRD1, KIAA1810 UniProt ID Q86TM6 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 Human IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background E3 ubiquitin-protein ligase synoviolin is an enzyme that in humans is encoded by the SYVN1 gene. This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. This gene and the mitochondrial ribosomal protein L49 gene use in their respective 3′ UTRs some of the same genomic sequence. Sequence analysis identified two transcript variants that encode different isoforms. Cellular location Endoplasmic reticulum, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:3000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: A549, 2: HeLa, 3: HEK-293, 4: HepG2Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 68 kDaObserved band size: 70 kDa IHC-P Tissue: Human liver cancerSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with