anti-sparc rat mab

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Recombinant Anti-SPARC Rat mAb Clone Number: JMMR-2261 Host: Rat Clonality: Recombinant Monoclonal Applications： WB Reactivity: Mouse Synonyms: Basement-membrane protein 40, BM-40, ON Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms Basement-membrane protein 40, BM-40, ON UniProt ID P09486 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Secreted Protein Acidic and Cysteine Rich (SPARC), also known as osteonectin or basement-membrane protein 40 (BM-40), is a secreted protein essential in the calcification of bone. Osteoblasts secrete SPARC during bone formation, with high levels found in immature bone compared to mature bone that is in homeostasis. SPARC is also secreted in adult mineralized tissues that have high turnover, such as osteoid and dentin, by cells other than osteoblasts. This includes bone marrow progenitor cells and hypertrophic chondrocytes but also endothelial cells and fibroblasts. SPARC contains both a collagen-binding domain and a hydroxyapatite (HA) binding region, so is thought to enhance mineralization by binding both collagen and HA crystals, causing the release of calcium ions. SPARC can bind to a number of different ECM proteins, particularly collagen, and may even influence the assembly of these proteins. Cellular location Basement membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rat IgGH&L (HRP Conjugate) Lysate: 1: C2C12, 2: Mouse placenta, 3: Mousetestis Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 35 kDa Observed band size: 40 kDa :
Recombinant Anti-SPARC Rat mAb Clone Number: JMMR-2261 Host: Rat Clonality: Recombinant Monoclonal Applications： WB Reactivity: Mouse Synonyms: Basement-membrane protein 40, BM-40, ON Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms Basement-membrane protein 40, BM-40, ON UniProt ID P09486 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Secreted Protein Acidic and Cysteine Rich (SPARC), also known as osteonectin or basement-membrane protein 40 (BM-40), is a secreted protein essential in the calcification of bone. Osteoblasts secrete SPARC during bone formation, with high levels found in immature bone compared to mature bone that is in homeostasis. SPARC is also secreted in adult mineralized tissues that have high turnover, such as osteoid and dentin, by cells other than osteoblasts. This includes bone marrow progenitor cells and hypertrophic chondrocytes but also endothelial cells and fibroblasts. SPARC contains both a collagen-binding domain and a hydroxyapatite (HA) binding region, so is thought to enhance mineralization by binding both collagen and HA crystals, causing the release of calcium ions. SPARC can bind to a number of different ECM proteins, particularly collagen, and may even influence the assembly of these proteins. Cellular location Basement membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rat IgGH&L (HRP Conjugate) Lysate: 1: C2C12, 2: Mouse placenta, 3: Mousetestis Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 35 kDa Observed band size: 40 kDa