anti-sox2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-SOX2 Rabbit mAb Clone Number: JRMR-10799-47B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms UniProt ID P48431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Sox2 is one of the essential factors regulating mammalian embryonic development. It is of central importance for the acquisition and maintenance of pluripotency. SOX2 is required for multiple steps in formation of the trachea, lung, and esophagus. During these processes, SOX2 is complementarily expressed with other transcription factors, for example, NKX2.1 and SOX9. SOX2 is an oncogene involved in the formation of cancers in the lung and esophagus, but exactly how SOX2 exerts its oncogenic function remains largely unknown. Cellular location Cell nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: NCCIT, 2: C6Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 34 kDaObserved band size: 34 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Human glioma, 2: F9Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 34 kDaObserved band size: 34 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa (Negative control), 2: NCCITProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 34 kDaObserved band size: 34 kDa IHC-P Tissue: Mouse stomachSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-SOX2 Rabbit mAb Clone Number: JRMR-10799-47B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms UniProt ID P48431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Sox2 is one of the essential factors regulating mammalian embryonic development. It is of central importance for the acquisition and maintenance of pluripotency. SOX2 is required for multiple steps in formation of the trachea, lung, and esophagus. During these processes, SOX2 is complementarily expressed with other transcription factors, for example, NKX2.1 and SOX9. SOX2 is an oncogene involved in the formation of cancers in the lung and esophagus, but exactly how SOX2 exerts its oncogenic function remains largely unknown. Cellular location Cell nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: NCCIT, 2: C6Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 34 kDaObserved band size: 34 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Human glioma, 2: F9Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 34 kDaObserved band size: 34 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa (Negative control), 2: NCCITProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 34 kDaObserved band size: 34 kDa IHC-P Tissue: Mouse stomachSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with