anti-2-hydroxyisobutyryllysine rabbit pab

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Anti-2-Hydroxyisobutyryllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: All Synonyms: Khib Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Khib UniProt ID / Immunogen 2-Hydroxyisobutyrylated lysine peptides MW (kDa) Multiple Specificity Anti-2-Hydroxyisobuyrylated Rabbit pAb detects proteins with 2-hydroxyisobutyrylated lysine residues. This pan antibody recognizes 2-hydroxyisobutyrylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 All Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation, and etc. Khib is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of Khib suggest that it is mechanistically and functionally different from histone lysine acetylation. Cellular location / Images Dot Blot Peptide amount: 4 ng, 20 ng, 100 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided below.Lane 1: Acetylated peptide library. Lane 2: Butyrylated peptide library. Lane 3: Crotonylated peptide library. Lane 4: Unmodified peptide library.Lane 5: 2-Hydroxyisobutyrylated peptide library. Lane 6: 2-Hydroxybutyrylated peptide library.Lane 7: β-Hydroxybutyrylated peptide library.Lane 8: 3-Hydroxyisobutyrylated peptide library.Lane 9: 4-Hydroxybutyrylated peptide library. WB Lysates: HeLa, MEF, S2, S.cerevisiaeProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: MultipleObserved band size: Multiple :
Anti-2-Hydroxyisobutyryllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: All Synonyms: Khib Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Khib UniProt ID / Immunogen 2-Hydroxyisobutyrylated lysine peptides MW (kDa) Multiple Specificity Anti-2-Hydroxyisobuyrylated Rabbit pAb detects proteins with 2-hydroxyisobutyrylated lysine residues. This pan antibody recognizes 2-hydroxyisobutyrylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 All Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation, and etc. Khib is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of Khib suggest that it is mechanistically and functionally different from histone lysine acetylation. Cellular location / Images Dot Blot Peptide amount: 4 ng, 20 ng, 100 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided below.Lane 1: Acetylated peptide library. Lane 2: Butyrylated peptide library. Lane 3: Crotonylated peptide library. Lane 4: Unmodified peptide library.Lane 5: 2-Hydroxyisobutyrylated peptide library. Lane 6: 2-Hydroxybutyrylated peptide library.Lane 7: β-Hydroxybutyrylated peptide library.Lane 8: 3-Hydroxyisobutyrylated peptide library.Lane 9: 4-Hydroxybutyrylated peptide library. WB Lysates: HeLa, MEF, S2, S.cerevisiaeProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: MultipleObserved band size: Multiple