anti-setdb1 mouse mab

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Recombinant Anti-SETDB1 Mouse mAb Clone Number: 10889 Host: Mouse Clonality: Monoclonal Applications： WB、IHC-P、ICC/IF Reactivity: Human, Mouse, Rat, Africangreenmonkey Synonyms: ESET, H3-K9-HMTase 4 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms ESET, H3-K9-HMTase 4 UniProt ID Q15047 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:1000 Human, Mouse, Rat, African green monkey IHC-P 1:50-1:100 Mouse ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone-lysine N-methyltransferase SETDB1 is an enzyme that in humans is encoded by the SETDB1 gene. SETDB1 is also known as KMT1E or H3K9 methyltransferase ESET. The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and ‘Enhancer of zeste’ proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HeLa, Jurkat, NIH/3T3, COS-7, PC-12 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 143 kDa Observed MW: 170 kDa IHC-P Tissue: Mouse skeletal muscle Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for :
Recombinant Anti-SETDB1 Mouse mAb Clone Number: 10889 Host: Mouse Clonality: Monoclonal Applications： WB、IHC-P、ICC/IF Reactivity: Human, Mouse, Rat, Africangreenmonkey Synonyms: ESET, H3-K9-HMTase 4 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms ESET, H3-K9-HMTase 4 UniProt ID Q15047 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:1000 Human, Mouse, Rat, African green monkey IHC-P 1:50-1:100 Mouse ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone-lysine N-methyltransferase SETDB1 is an enzyme that in humans is encoded by the SETDB1 gene. SETDB1 is also known as KMT1E or H3K9 methyltransferase ESET. The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and ‘Enhancer of zeste’ proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HeLa, Jurkat, NIH/3T3, COS-7, PC-12 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 143 kDa Observed MW: 170 kDa IHC-P Tissue: Mouse skeletal muscle Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for