anti-senp1 rabbit mab

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Recombinant Rabbit Monoclonal Anti-SENP1 Rabbit mAb Clone Number: JMMR-4058-VH Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: / Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID Q9P0U3 Immunogen Peptide MW (kDa) 73 Specificity / Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background SENP1 is a member of the sentrin/SUMO-specific protease (SENP) family. SENP1 localizes to the nucleoplasm and catalyzes the release of SUMO1, SUMO2, and SUMO3 monomers from sumoylated substrates. SENP1 has been reported to be responsible for intracellular SUMO homeostasis in the control of normal cellular function. The removal of sumoylation by SENP1 from many important target proteins, such as HDAC1, HIF-1α, , Stat5, p300, Elk-1, and SirT1, leads to the regulation of the related biological pathways. SENP1-induced desumoylation ofHIF-1α stabilizes the target during hypoxia, activating downstream VEGF expression and angiogenesis. SENP1 desumoylates Stat5 andcontributes to Stat5 acetylation and subsequent signaling during normal lymphocyte development. Under stress conditions, SENP1 interactswith and inactivates SirT1 by desumoylation, protecting cells from apoptosis. SENP1 has been reported to target the progesterone andandrogen receptors, either directly or indirectly through HDAC1, thereby upregulating their transcriptional function and potentially affectingreceptor-related cancer progression Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP)Lysate: 1:HeLa, 2: HEK-293, 3: PANC-1, 4: F9, 5: NIH-3T3, 6: C6Protein loading quantity: 20 μgExposure time: 3 sPredicted band size: 73 kDaObserved band size: 73 kDa :
Recombinant Rabbit Monoclonal Anti-SENP1 Rabbit mAb Clone Number: JMMR-4058-VH Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: / Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID Q9P0U3 Immunogen Peptide MW (kDa) 73 Specificity / Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background SENP1 is a member of the sentrin/SUMO-specific protease (SENP) family. SENP1 localizes to the nucleoplasm and catalyzes the release of SUMO1, SUMO2, and SUMO3 monomers from sumoylated substrates. SENP1 has been reported to be responsible for intracellular SUMO homeostasis in the control of normal cellular function. The removal of sumoylation by SENP1 from many important target proteins, such as HDAC1, HIF-1α, , Stat5, p300, Elk-1, and SirT1, leads to the regulation of the related biological pathways. SENP1-induced desumoylation ofHIF-1α stabilizes the target during hypoxia, activating downstream VEGF expression and angiogenesis. SENP1 desumoylates Stat5 andcontributes to Stat5 acetylation and subsequent signaling during normal lymphocyte development. Under stress conditions, SENP1 interactswith and inactivates SirT1 by desumoylation, protecting cells from apoptosis. SENP1 has been reported to target the progesterone andandrogen receptors, either directly or indirectly through HDAC1, thereby upregulating their transcriptional function and potentially affectingreceptor-related cancer progression Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP)Lysate: 1:HeLa, 2: HEK-293, 3: PANC-1, 4: F9, 5: NIH-3T3, 6: C6Protein loading quantity: 20 μgExposure time: 3 sPredicted band size: 73 kDaObserved band size: 73 kDa