anti-runx2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-RUNX2 Rabbit mAb Clone Number: JRMR-10386-2-H4L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: AML3, CBFA1, OSF2, PEBP2A Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms AML3, CBFA1, OSF2, PEBP2A UniProt ID Q13950 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background RUNX2 is a member of the RUNX family of transcription factors. It is involved in osteoblast differentiation and skeletal morphogenesis. RUNX2 regulates the transcription of various genes, including osteopontin, bone sialoprotein, and osteocalcin, via binding to the core site of the enhancers or promoters. RUNX2 is crucial for the maturation of osteoblasts and both intramembranous and endochondral ossification. Mutations in the corresponding RUNX2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). RUNX2 is also abnormally expressed in various human cancers including prostate cancer and breast cancer. It plays an important role in migration, invasion, and bone metastasis of prostate and breast cancer cells. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: LnCAP (Negative control), 2: MDA-MB-231, 3: PC-3 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 57 kDa Observed band size: 64 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: Saos-2, 2: C2C12 Protein loading quantity: 20 μg Exposure time: 30 seconds/ 60 secondsPredicted band size: 57 kDa Observed band size: 64 kDa :
Recombinant Rabbit Monoclonal Anti-RUNX2 Rabbit mAb Clone Number: JRMR-10386-2-H4L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: AML3, CBFA1, OSF2, PEBP2A Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms AML3, CBFA1, OSF2, PEBP2A UniProt ID Q13950 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background RUNX2 is a member of the RUNX family of transcription factors. It is involved in osteoblast differentiation and skeletal morphogenesis. RUNX2 regulates the transcription of various genes, including osteopontin, bone sialoprotein, and osteocalcin, via binding to the core site of the enhancers or promoters. RUNX2 is crucial for the maturation of osteoblasts and both intramembranous and endochondral ossification. Mutations in the corresponding RUNX2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). RUNX2 is also abnormally expressed in various human cancers including prostate cancer and breast cancer. It plays an important role in migration, invasion, and bone metastasis of prostate and breast cancer cells. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: LnCAP (Negative control), 2: MDA-MB-231, 3: PC-3 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 57 kDa Observed band size: 64 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: Saos-2, 2: C2C12 Protein loading quantity: 20 μg Exposure time: 30 seconds/ 60 secondsPredicted band size: 57 kDa Observed band size: 64 kDa