anti-plxnb2 mouse mab

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Recombinant Anti-PLXNB2 Mouse mAb Clone Number: 13696 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: KIAA0315 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms KIAA0315 UniProt ID O15031 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Plays a role in glutamatergic synapse development and is required for SEMA4A-mediated excitatory synapse development. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at ‘Tyr-1248’. Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development. Regulates the migration of cerebellar granule cells in the developing brain. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. May modulate the activity of RAC1 and CDC42. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, MCF-7Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 240/150 kDaObserved band size: 240/150 kDa ICC/IF Cell line: MCF7Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Anti-PLXNB2 Mouse mAb Clone Number: 13696 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: KIAA0315 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms KIAA0315 UniProt ID O15031 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Plays a role in glutamatergic synapse development and is required for SEMA4A-mediated excitatory synapse development. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at ‘Tyr-1248’. Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development. Regulates the migration of cerebellar granule cells in the developing brain. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. May modulate the activity of RAC1 and CDC42. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, MCF-7Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 240/150 kDaObserved band size: 240/150 kDa ICC/IF Cell line: MCF7Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with